La chromatographie en phase inverse utilisant des réactifs d’appariement d’ions tels que le TFA et l’acide formique sépare de manière très résolutive les mélanges peptidiques complexes (par exemple, les digestions tryptiques de protéines ou les longues séquences de peptides synthétiques) dont les séquences peuvent différer d’un seul acide aminé. L’hydrophobicité du peptide détermine l’ordre d’élution, les peptides les moins hydrophobes éluant en premier. Des facteurs tels que la composition des particules (silice ou hybrides), la taille des pores (130 Å ou 300 Å), et la densité du ligand, ainsi que les conditions de séparation (par exemple, durée du gradient, température de la colonne, débit) jouent tous un rôle important dans l’obtention de la séparation attendue. Comme pour les autres séparations, lorsque l’on utilise des particules de plus petite taille pour les modes UHPLC et UPLC, les colonnes offrent une meilleure résolution
France
More than 200 combinations of column configurations and chemistries, UPLC Columns combine faster separations with higher resolution and sensitivities by harnessing the full potential of small particles. All Waters UPLC Columns are designed, tested, and guaranteed for routine use in applications up to 18,000 psi (1241 bar).
Demander un devisFrance
nanoACQUITY Columns and trap columns are the previous generation of columns for nano- and microflow LC-MS applications. They are being replaced by the new generation of ACQUITY UPLC M-Class columns: Higher pressure rating (15,000 psi/1,034 bar) enable higher flow rates and thus shorter run times and higher sample turnover. M-Class Columns are compatible with nanoACQUITY UPLC systems.
Demander un devisFrance
Waters nano- and microflow LC-MS columns are specifically designed for low-dispersion nano LC systems. The selected stationary phases fully leverage the separation power of sub-2-µm particle technology. This delivers the efficiency and selectivity required for complex separations such as discovery of low abundance species, peptide mapping, protein identification, and biomarker discovery. Leverage the efficiency of sub-2-µm particle technology at high flow rates Stationary phases with a variety of characteristics suitable for biomolecule separations Easy interface with LC-MS systems
Demander un devisFrance
Columns designed for reliable nano, capillary, and microscale UPLC separations Waters nano columns offer purposefully-designed hardware to meet the different demands of today's nanoflow LC and LC-MS separations. The UPLC Column delivers superior chromatographic resolution and fully leverages the separation power of small sub-2-µm particles. The selected stationary phases for nano-LC and trapping columns provide an advantage developing fast and efficient separations for complex peptide and protein separations and other sample limited analyses. ACQUITY UPLC M-Class Columns (rated for 15,000 psi [1,000 bar]) allow for faster separations and are compatible with the nanoACQUITY UPLC system. The iKey Separation Device is an enabling technology behind the ionKey/MS System. It eliminates the need for traditional fittings and greatly simplifies the microflow-LC user experience.
Demander un devis