Accessoires

High pore volume HPLC particles do not possess the mechanical stability necessary to withstand the high pressures inherent in UPLC® separations. Waters material scientists addressed this challenge by developing a silica particle designed for high mechanical stability with the appropriate morphology to provide long UPLC column lifetimes and high UPLC efficiencies at high pressures. The 1.8 µm High Strength Silica (HSS) particle is the first and only 100% silica particle designed, tested and intended for use in applications up to 15,000 psi (1034 bar). HSS particle technology is also available in HPLC particle sizes (3.5 and 5 µm) in the HSS family of HPLC columns, enabling seamless transfer between HPLC and UPLC technology platforms.

The 1.7 µm Charged Surface Hybrid (CSH™) particle is Waters third generation hybrid particle technology. Based on Waters Ethylene Bridged Hybrid (BEH) particle technology, CSH particles incorporate a low level surface charge that improves sample loadability and peak asymmetry in low-ionic-strength mobile phases, while maintaining the mechanical and chemical stability inherent in the BEH particle technology. The advantages of CSH Technology UPLC chromatography columns include: Superior peak shape for basic compounds Increased loading capacity Rapid column equilibration after changing mobile-phase pH Improved batch-to-batch reproducibility Exceptional stability at low and high pH Click here for Waters new CSH130 columns for peptide separations. CSH particle technology is also available in HPLC particle sizes (3.5 and 5 µm) in the XSelect™ family of HPLC columns, enabling seamless transfer between HPLC and UPLC® technology platforms.

The 1.7 µm Ethylene Bridged Hybrid [BEH] particle is one of the key enablers behind UPLC Technology. It is available in three different pore sizes [130Å, 200Å, and 300Å] and several bonded phases for reversed-phase and hydrophilic interaction chromatography and is applicable from small molecule to large biopharmaceutical analysis. Due to the intrinsic chemical stability of hybrid particle technology, a wider usable pH range [pH 1-12] can be employed, enabling a versatile and robust separation technology for method development. BEH particle technology is available in HPLC particle sizes [2.5, 3.5, 5 and 10 µm] in the XBridge family of HPLC columns, enabling seamless transfer between HPLC and UPLC technology platforms. ACQUITY UPLC BEH C18 and C8 Columns Providing unprecedented levels of peak asymmetry, efficiency, and chemical stability, the 1.7 µm ACQUITY UPLC BEH C18 and C8 Columns are versatile, high-performance separation columns suitable

La purification HPLC à l’échelle du laboratoire représente nombre de défis pour les chromatographistes, dont l’un des plus complexes repose sur les colonnes préparatives elles-mêmes. Les variabilités liées aux performances et aux durées de vie inter colonnes peuvent entraîner des pertes d’échantillons, la répétition inutile d’injections, ou encore une piètre transposition lors du changement d’échelle. Conscients de ces limitations, nous avons étudié pendant trois ans tous les aspects des procédés de remplissage, ainsi que de la conception de colonnes. Lancée en 2003, et éprouvée dans les laboratoires depuis maintenant plus de dix ans, notre technologie OBD (Optimum Bed Density) brevetée constitue l’aboutissement de toutes ces recherches.